P-96: Survey of In Vitro Effect of Noscapine on Nitric Oxide Secretion of Human Endometrial Stromal Cell
Authors
Abstract:
Background: Noscapine is cough suppressant drug with cell inhibitory effect. It is introduced for anti-cancer treatment. The aim of present study was to determine in vitro noscapine effect on nitric oxide (NO) secretion by human endometrial stromal cells. Materials and Methods: Human endometrial biopsies (n=8) were obtained in sterile condition and were chopped mechanically and digested by enzymatic (collagenase, 2 mg/ml) methods. Stromal cells were harvested after using cell strainer (70 and 100 μm) and centrifugation. The cells were divided into four groups: control, 10, 25, 50 and 100 μM noscapine concentration for three culture periods (24, 48, 72 hours), Nitric oxide secretion were assessed by Geriss assay. Data was analyzed by one way ANOVA and p< 0.05 was considered significant. Results: Difference between NO amount were significant in control, 10, 25, 50 and 100 μM noscapine concentration were 35.14, 33.79, 20.7, 19.69 and 18.75 μM at 24-hour culture period (p=.009), 56.22, 53.54,26.84. 25.03 and 22.35 μM at 48-hour(p=0.01), and 73.99, 63.42, 33.33, 32.77 and 30.6 μM at 72-hour (p=0.037) respectively. Conclusion: Noscapine decreased endometrial stromal cells NO secretion in dose dependant manner.
similar resources
P-113: Survey of In Vitro Effect of Resveratrol on Nitric Oxide Secretion of Human Endometrial Epithelial Cells
Background: Resveratrol is a natural polyphe- nolic compound, synthesized by plants as a phytoalexin and protects against ultraviolet radiation and fungal infection. Nitric oxide is formed from L-arginine through nitric oxide synthases (NOS), a group of enzymes that structurally resemble cytochrome P-450 reductase. In females, circulating NO is increased during follicle development and decrease...
full textP-99: Survey of Noscapine Effect on Apoptosis of Human Endometrial Stromal Cells: An In Vitro Study
Background: Noscapine is a water soluble alkaloid with antitussive property and very less toxicity. It is obtained from the Papaveraceae family. Recently, new pharmacological properties such as anticancer, anti stroke and anti-angiogenic effects are introduced. There is no scientific report on noscapine effect on reproductive system. The aim of present study was to determine in vitro effect of ...
full textSildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro
full text
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
P-156: Survey of In Vitro Effect of Resveratrol on Apoptosis of Human Endometrial Epithelial Cells
Background: In apoptosis is physiological cell death which eliminates injured and old cells. Any disruption in apoptosis, leads to disease. Resveratrol is a natural polyphenol with estrogen-like, antioxidant and antiinflammatory properties; also it showed cell prolifratory and inhibitory effect. The aim of present study was to determine resveratrol effect on apoptosis of human endometrial epith...
full text: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
My Resources
Journal title
volume 7 issue 3
pages 74- 74
publication date 2013-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023